Proteintech, 51068-1-AP, ERK1/2 Polyclonal antibody

Size: 20ul / 150ul The ERK1/2 (51068-1-AP) by Proteintech is a Polyclonal antibody targeting ERK1/2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 51068-1-AP targets ERK1/2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, mouse brain tissue, Jurkat cells, NIH/3T3 cells, mouse brain cells, rat brain cells, rat skin tissue Positive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, duck Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase 1 Calculated Molecular Weight: 360 aa, 41 kDa Observed Molecular Weight: 38-44 kDa GenBank Accession Number: BC017832 Gene Symbol: ERK2 Gene ID (NCBI): 5594 RRID: AB_2250380 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P28482 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.