Proteintech, 55011-1-AP, HTRA1 Polyclonal antibody

Size: 20ul / 150ul HTRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA 55011-1-AP targets HTRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, HAP1 cells, rat brain tissue Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: HtrA serine peptidase 1 Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 48-50 kDa GenBank Accession Number: NM_002775 Gene Symbol: HTRA1 Gene ID (NCBI): 5654 RRID: AB_10859830 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92743 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.