Proteintech, 55027-1-AP, CSPG4 Polyclonal antibody

Size: 20ul / 150ul The CSPG4 (55027-1-AP) by Proteintech is a Polyclonal antibody targeting CSPG4 in WB, ELISA applications with reactivity to human, mouse, rat samples 55027-1-AP targets CSPG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A375 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information CSPG4, also named as HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16 and NG2, is a proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. CSPG4 may inhibit neurite outgrowth and growth cone collapse during axon regeneration. It is cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. CSPG4 may regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. It modulates the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. CSPG4 functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. It promotes retraction fiber formation and cell polarization through Rho GTPase activation and stimulates alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. CSPG4 activates FAK and ERK1/ERK2 signaling cascades. The antibody is specific to CSPG4. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: chondroitin sulfate proteoglycan 4 Calculated Molecular Weight: 251 kDa Observed Molecular Weight: 240-250 kDa, 450kDa GenBank Accession Number: NM_001897 Gene Symbol: CSPG4 Gene ID (NCBI): 1464 RRID: AB_10791888 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6UVK1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.