Proteintech, 55047-1-AP, HuC Polyclonal antibody

Size: 20ul / 150ul The HuC (55047-1-AP) by Proteintech is a Polyclonal antibody targeting HuC in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 55047-1-AP targets HuC in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, Neuro-2a cells Positive IP detected in: Neuro-2a cells Positive IHC detected in: human lung cancer tissue, human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information HUC , belongs to the RRM elav family. It binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. ELAVL3 may also bind poly-A tracts via RRM 3. It is involved in neuronal differentiation and maintenance. The antibody has no cross reaction to other ELAVL members. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 36-38 kDa GenBank Accession Number: NM_001420 Gene Symbol: HuC Gene ID (NCBI): 1995 RRID: AB_10859256 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14576 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.