Proteintech, 55060-1-AP, PYCR2-specific Polyclonal antibody

Size: 20ul / 150ul The PYCR2-specific (55060-1-AP) by Proteintech is a Polyclonal antibody targeting PYCR2-specific in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 55060-1-AP targets PYCR2-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, L02 cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody is specific to PYCR2 and has no cross reaction to PYCR1. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: pyrroline-5-carboxylate reductase family, member 2 Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 27-31 kDa, 20 kDa GenBank Accession Number: NM_013328 Gene Symbol: PYCR2 Gene ID (NCBI): 29920 RRID: AB_10888635 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96C36 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.