Proteintech, 55082-1-AP, MTCO3 Polyclonal antibody

Size: 20ul / 150ul The MTCO3 (55082-1-AP) by Proteintech is a Polyclonal antibody targeting MTCO3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 55082-1-AP targets MTCO3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue, C2C12 cells, mouse liver tissue, rat kidney tissue, rat liver tissue Positive IHC detected in: mouse cerebellum tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information MTCO3 (cytochrome c oxidase subunit III) is a mitochondrial DNA encoded subunit of respiratory Complex IV which is located within the mitochondrial inner membrane and is the final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. The predicted MW of MTCO3 is 30 kDa, while it had been observed as 23 kDa in some papers (PMID: 17557076). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cytochrome c oxidase III Calculated Molecular Weight: 30 kDa Observed Molecular Weight: 32-35 kDa GenBank Accession Number: YP_003024032 Gene Symbol: MTCO3 Gene ID (NCBI): 4514 RRID: AB_2881265 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P00414 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.