Proteintech, 55138-1-AP, PRKG2 Polyclonal antibody

Size: 20ul / 150ul The PRKG2 (55138-1-AP) by Proteintech is a Polyclonal antibody targeting PRKG2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 55138-1-AP targets PRKG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human colon tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: C6 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PRKG2, also named as PRKGR2 and cGK2, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and cGMP subfamily. It catalyses the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to PRKG2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protein kinase, cGMP-dependent, type II Calculated Molecular Weight: 87 kDa Observed Molecular Weight: 85-90 kDa GenBank Accession Number: NM_006259 Gene Symbol: PRKG2 Gene ID (NCBI): 5593 RRID: AB_10804353 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13237 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.