Proteintech, 55190-1-AP, ATGL Polyclonal antibody

Size: 20ul / 150ul The ATGL (55190-1-AP) by Proteintech is a Polyclonal antibody targeting ATGL in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 55190-1-AP targets ATGL in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, 3T3-L1 cells, A431 cells, HeLa cells, human heart tissue, mouse liver tissue, mouse testis tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, rat liver tissue Positive IP detected in: mouse testis tissue Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, monkey, chicken, zebrafish, bovine, sheep Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: patatin-like phospholipase domain containing 2 Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 45-55 kDa GenBank Accession Number: NM_020376 Gene Symbol: ATGL Gene ID (NCBI): 57104 RRID: AB_11182818 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96AD5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.