Proteintech, 55210-1-AP, ACADM Polyclonal antibody

Size: 20ul / 150ul The ACADM (55210-1-AP) by Proteintech is a Polyclonal antibody targeting ACADM in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 55210-1-AP targets ACADM in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, K-562 cells, HepG2 cells, rat heart tissue, MDA-MB-453s cells, mouse liver tissue, rat liver tissue, mouse kidney tissue, rat kidney tissue Positive IHC detected in: human liver tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: NM_000016 Gene Symbol: ACADM Gene ID (NCBI): 34 RRID: AB_10837361 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P11310 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.