Proteintech, 55214-1-AP, GDI1 Polyclonal antibody

Size: 20ul / 150ul The GDI1 (55214-1-AP) by Proteintech is a Polyclonal antibody targeting GDI1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 55214-1-AP targets GDI1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SH-SY5Y cells, human brain tissue, mouse brain tissue Positive IP detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55kda protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034). Defects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41). Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48). The antibody is specific to GDI1. And it has no cross reaction to GDI2. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: GDP dissociation inhibitor 1 Calculated Molecular Weight: 51 kDa Observed Molecular Weight: 51-55 kDa GenBank Accession Number: NM_001493 Gene Symbol: GDI1 Gene ID (NCBI): 2664 RRID: AB_10858798 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P31150 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.