Proteintech, 55222-1-AP, HCN1 Polyclonal antibody

Size: 20ul / 150ul The HCN1 (55222-1-AP) by Proteintech is a Polyclonal antibody targeting HCN1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 55222-1-AP targets HCN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human heart tissue, human cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information HCN1, also named as BCNG1, belongs to the potassium channel HCN family. It hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. HCN1 contributes to the native pacemaker currents in heart (If) and in neurons (Ih). It is activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. HCN1 may mediate responses to sour stimuli. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 1 Calculated Molecular Weight: 99 kDa Observed Molecular Weight: 99-120 kDa GenBank Accession Number: NM_021072 Gene Symbol: HCN1 Gene ID (NCBI): 348980 RRID: AB_11182929 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O60741 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.