Proteintech, 55227-1-AP, SPTA1 Polyclonal antibody

Size: 20ul / 150ul The SPTA1 (55227-1-AP) by Proteintech is a Polyclonal antibody targeting SPTA1 in WB, IF-P, FC (Intra), ELISA applications with reactivity to human, rat samples 55227-1-AP targets SPTA1 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: rat red blood cells, human blood Positive IF-P detected in: mouse lung tissue Positive FC (Intra) detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Alpha 1 Spectrin also known as SPTA1, EL2, belongs to the spectrin family. Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Mutations in SPTA1 result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3(PMID: 1541680, 8941647). Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: spectrin, alpha, erythrocytic 1 (elliptocytosis 2) Calculated Molecular Weight: 280 kDa Observed Molecular Weight: 280 kDa GenBank Accession Number: NM_003126 Gene Symbol: SPTA1 Gene ID (NCBI): 6708 RRID: AB_3086436 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P02549 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.