Proteintech, 55238-1-AP, Caspase 12 Polyclonal antibody

Size: 20ul / 150ul The Caspase 12 (55238-1-AP) by Proteintech is a Polyclonal antibody targeting Caspase 12 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 55238-1-AP targets Caspase 12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, multi-cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human prostate hyperplasia tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases. Specification Tested Reactivity: human Cited Reactivity: human, pig, hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: caspase 12 (gene/pseudogene) Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 36-42 kDa, 50 kDa GenBank Accession Number: NR_000035 Gene Symbol: Caspase 12 Gene ID (NCBI): 120329 RRID: AB_10892670 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.