Proteintech, 55279-1-AP, CTAGE5 Polyclonal antibody

Size: 20ul / 150ul The CTAGE5 (55279-1-AP) by Proteintech is a Polyclonal antibody targeting CTAGE5 in WB, IHC, ELISA applications with reactivity to human samples 55279-1-AP targets CTAGE5 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, HepG2 cells, human brain tissue, PC-3 cells, human testis tissue Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241). This antibody is specific to CTAGE5. It can recognize all the isoforms except isoform 5B. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: CTAGE family, member 5 Calculated Molecular Weight: 92 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: NM_005930 Gene Symbol: CTAGE5 Gene ID (NCBI): 4253 RRID: AB_11183044 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96PC5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.