Proteintech, 55309-1-AP, MAT2A Polyclonal antibody

Size: 20ul / 150ul The MAT2A (55309-1-AP) by Proteintech is a Polyclonal antibody targeting MAT2A in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 55309-1-AP targets MAT2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SGC-7901cells, NIH/3T3 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, human kidney tissue, mouse liver tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MAT2A, also named as AMS2, MATA2 and AdoMet synthase 2, belongs to the AdoMet synthase family. It catalyzes the formation of S-adenosylmethionine from methionine and ATP. This antibody is specific to MATA2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: methionine adenosyltransferase II, alpha Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 47 kDa, 49 kDa GenBank Accession Number: NM_005911 Gene Symbol: MAT2A Gene ID (NCBI): 4144 RRID: AB_2881303 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P31153 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.