Proteintech, 55354-1-AP, JMJD3 Polyclonal antibody

Size: 20ul / 150ul The JMJD3 (55354-1-AP) by Proteintech is a Polyclonal antibody targeting JMJD3 in WB, ELISA applications with reactivity to human samples 55354-1-AP targets JMJD3 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Background Information JMJD3, also known as KDM6B, is a 1643 amino acid protein, which belongs to the UTX family. JMJD3 is a Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed:17825402, PubMed:17851529, ). JMJD3 demethylates trimethylated and dimethylated H3 'Lys-27', so it Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed:17851529). Histone demethylases are epigenetic actors with a crucial role in cancer by acting as suppressors of tumors or as oncogenes. JMJD3 and UTX (ubiquitously transcribed tetratricopeptide repeat, X chromosome) are transcription activators, being specific H3K27me3 demethylases. JMJD3 is involved in many cellular process such as development, differentiation, senescence and aging by p16, p53 and RB pathways and finally inflammation. Depending on cancer type, JMJD3 expression is increased (prostate and breast cancers, melanoma, gliomas, renal cell carcinoma or decreased (lung, liver, pancreatic, colon and colorectal cancers. This role in carcinogenesis has allowed the development of "epidrugs" to modulate JMJD3 expression (PMID: 29805743 ). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: jumonji domain containing 3, histone lysine demethylase Calculated Molecular Weight: 177 kDa Observed Molecular Weight: 177 kDa GenBank Accession Number: NM_001080424 Gene Symbol: JMJD3 Gene ID (NCBI): 23135 RRID: AB_2752227 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15054 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.