Proteintech, 55367-1-AP, EVC2 Polyclonal antibody

Size: 20ul / 150ul The EVC2 (55367-1-AP) by Proteintech is a Polyclonal antibody targeting EVC2 in WB, IHC, ELISA applications with reactivity to Mouse, Human samples 55367-1-AP targets EVC2 in WB, IHC, ELISA applications and shows reactivity with Mouse, Human samples. Tested Applications Positive WB detected in: mouse lung tissue Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information EVC2 (also known as LIMBIN) is close proximity in divergent orientation to EVC and mutations in both human genes lead to the chondrodysplasia Ellis-van Creveld (EvC) syndrome. Typical symptoms of EvC include shorter limbs and ribs, postaxial polydactyly, dysplastic nails, and cardiovascular defects (PMID: 28081373). Additionally, EvC syndrome has been characterized as a ciliopathy because the EVC and EVC2 proteins are intracellularly localized at the bottom of the primary cilium, where they form a heterotrimeric protein complex with SMO and transduce Hedgehog(Hh) signaling (PMID: 30410447). EVC2 is expressed in the heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. EVC2 has 3 isoforms with the molecular mass of 140-148 kDa. Specification Tested Reactivity: Mouse, Human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Ellis van Creveld syndrome 2 Calculated Molecular Weight: 148 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: NM_147127 Gene Symbol: EVC2 Gene ID (NCBI): 132884 RRID: AB_3086445 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86UK5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.