Proteintech, 55380-1-AP, PYGB Polyclonal antibody

Size: 20ul / 150ul The PYGB (55380-1-AP) by Proteintech is a Polyclonal antibody targeting PYGB in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 55380-1-AP targets PYGB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HeLa cells, MCF-7 cells, U-251 cells Positive IHC detected in: mouse heart tissue, mouse brain tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information PYGB belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. This antibody is specific to PYGB. It has no cross reaction to PYGM and PYGL. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: phosphorylase, glycogen; brain Calculated Molecular Weight: 97 kDa Observed Molecular Weight: 97-105 kDa GenBank Accession Number: NM_002862 Gene Symbol: PYGB Gene ID (NCBI): 5834 RRID: AB_11182159 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P11216 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.