Proteintech, 55392-1-AP, PGAP1 Polyclonal antibody

Size: 20ul / 150ul The PGAP1 (55392-1-AP) by Proteintech is a Polyclonal antibody targeting PGAP1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 55392-1-AP targets PGAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human testis tissue, HEK-293 cells, MCF-7 cells, mouse testis tissue Positive IHC detected in: human testis tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PGAP1 belongs to the GPI inositol-deacylase family. It is involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi. PGAP1 is the GPI inositol-deacylase (PMID:14734546). This antibody is specific to PGAP1. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: post-GPI attachment to proteins 1 Calculated Molecular Weight: 105 kDa Observed Molecular Weight: 49-53 kDa, 85-90 kDa GenBank Accession Number: NM_024989 Gene Symbol: PGAP1 Gene ID (NCBI): 80055 RRID: AB_11232412 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q75T13 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.