Proteintech, 55405-1-AP, NR4A3 Polyclonal antibody

Size: 20ul / 150ul The NR4A3 (55405-1-AP) by Proteintech is a Polyclonal antibody targeting NR4A3 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 55405-1-AP targets NR4A3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse thymus tissue, mouse spleen tissue Positive IF/ICC detected in: ROS1728 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Nuclear receptor 4A3 (NR4A3, also known as NOR-1), NR4A1 and NR4A2 are TFs belonging to NR4A orphan nuclear receptor subfamily. The role of all three NR4A members in regulating gene expression involved in cell proliferation, differentiation, apoptosis, inflammation and metabolism in cardiovascular diseases has been widely studied. In osteoblasts, NR4A receptor has been proved to regulate the expression and differentiation of osteoblast genes. It is reported that NR4A receptor is highly expressed in human coronary atherosclerotic lesions, and NR4A3 deficiency can reduce the proliferation and migration of VSMCs and the formation of neointima after vascular injury. Therefore, NR4A3 may play an important role in mediating phenotypic transformation of VSMC (PMID: 38629274). It is expected to be located in nucleus. The protein has three isforms, isoform alpha is highly expressed in skeletal muscle. Isoform beta is highly expressed in skeletal muscle and low expressed in fetal brain and placenta. The calculated molecular weight of NR4A3 is 68 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: nuclear receptor subfamily 4, group A, member 3 Calculated Molecular Weight: 70 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: NM_006981 Gene Symbol: NR4A3 Gene ID (NCBI): 8013 RRID: AB_2881322 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92570 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.