Proteintech, 55408-1-AP, HIPK2 Polyclonal antibody

Size: 20ul / 150ul The HIPK2 (55408-1-AP) by Proteintech is a Polyclonal antibody targeting HIPK2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 55408-1-AP targets HIPK2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse kidney tissue Positive IHC detected in: mouse brain tissue, human gliomas tissue, human renal cell carcinoma tissue, mouse heart tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information HIPK2 is a serine/threonine kinase participating in transcriptional regulation, growth control, and apoptosis. The kinase contributes to the regulation of remarkably diverse pathways such as p53 activation or Wnt signaling. There is very low levels of HIPK2 protein expression in several thyroid tumors and the combination of HIPK2 and Gal-3,would be helpful in ameliorating the preoperative recognition of thyroid cancer. HIPK2 may represent a new tumour suppressor gene for these types of cancers and may constitute a new potential promising diagnostic marker and therapeutic target.(PMID:21698151). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: homeodomain interacting protein kinase 2 Calculated Molecular Weight: 131 kDa Observed Molecular Weight: 131 kDa, 101 kDa GenBank Accession Number: NM_022740 Gene Symbol: HIPK2 Gene ID (NCBI): 28996 RRID: AB_2881323 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H2X6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.