Proteintech, 55414-1-AP, BCL11B Polyclonal antibody

Size: 20ul / 150ul The BCL11B (55414-1-AP) by Proteintech is a Polyclonal antibody targeting BCL11B in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 55414-1-AP targets BCL11B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, mouse thymus tissue, MOLT-4 cells Positive IP detected in: Jurkat cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: B-cell CLL/lymphoma 11B (zinc finger protein) Calculated Molecular Weight: 96 kDa Observed Molecular Weight: 116 kDa GenBank Accession Number: NM_022898 Gene Symbol: BCL11B Gene ID (NCBI): 64919 RRID: AB_11182609 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9C0K0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.