Proteintech, 55457-1-AP, UBE2E1 Polyclonal antibody

Size: 20ul / 150ul The UBE2E1 (55457-1-AP) by Proteintech is a Polyclonal antibody targeting UBE2E1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 55457-1-AP targets UBE2E1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information UBE2E1 is a class III E2 ubiquitin conjugating enzyme that belongs to the UBE2E family of E2s, comprising UBE2E1/UBCH6, UBE2E2/UBCH8, and UBE2E3/UBCH9. UBE2E family members share a highly conserved UBC domain but are distinguished from one another by their unique N-terminal extensions. UbE2E1 contains a 50-residue unstructured N-terminal extension rich in serine and lysine residues, which has been shown to interact with USP7 and is essential for USP7-mediated UbE2E1 stabilization. In addition to its ubiquitin conjugating activity, UBE2E1 can also act as an ISG15 conjugating enzyme in vitro, although this activity has not been shown to occur in vivo. UBE2E1 can be covalently modified with either ISG15 or ubiquitin, both of which interfere with the ubiquitin conjugating activity of UBE2E1. UBE2E1 has also been reported to monoubiquitinate histone H2A at K119 in concert with the PRC1 E3 ligase complex. Full-length UBE2E1 is reported to be a dimer, with dimerization mediated by interaction of residues 21-27 from one molecule with the core domain of another molecule, as well as by contacts between a1 of the two interacting molecules. (PMID: 28073915, PMID: 30282802, PMID: 23871895) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast) Calculated Molecular Weight: 21 kDa Observed Molecular Weight: 21-27 kDa GenBank Accession Number: NM_003341 Gene Symbol: UBE2E1 Gene ID (NCBI): 7324 RRID: AB_2918357 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P51965 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.