Proteintech, 55463-1-AP, Phospholipase C epsilon 1 Polyclonal antibody

Size: 20ul / 150ul The Phospholipase C epsilon 1 (55463-1-AP) by Proteintech is a Polyclonal antibody targeting Phospholipase C epsilon 1 in IHC, ELISA applications with reactivity to human samples 55463-1-AP targets Phospholipase C epsilon 1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human kidney tissue, human oesophagus cancer tissue, human stomach cancer tissue, human colon tissue, human colon cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Phospholipase C epsilon 1 (PLCE1) is also named as KIAA1516, PLCE and PPLC. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. PLCE1, which mediates small GTPases of the Ras superfamily through the activity of its Ras guanine exchange factor, is a bifunctional enzyme (PMID: 31867717). As the effector of heterotrimer and small G protein, it is involved in regulating cell growth, cell survival, T-cell activation, and actin organization (PMID: 32238860). PLCE1 is identified as a member of phospholipase family, and PLCE1 was upregulated in LPS-induced pneumonia cells (PMID: 35527658). PLCE1 is involved in the regulation of Rho GTPases and MAPKs in podocytes, and PLCE1 interacts with NCK2 but not with NCK1 (PMID: 32238860). PLCE1 is a multifunctional enzyme exhibiting phospholipase as well as RasGEF activities (PMID: 11022047). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: phospholipase C, epsilon 1 Calculated Molecular Weight: 257 kDa GenBank Accession Number: NM_016341 Gene Symbol: PLCE1 Gene ID (NCBI): 51196 RRID: AB_3086452 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P212 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.