Proteintech, 60003-2-Ig, MYC tag Monoclonal antibody

Size: 150ul The MYC tag (60003-2-Ig) by Proteintech is a Monoclonal antibody targeting MYC tag in WB, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to recombinant protein samples 60003-2-Ig targets MYC tag in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: Transfected HEK-293T cells, recombinant proein Positive IP detected in: Transfected HEK-293 cells Positive IF/ICC detected in: Transfected HEK-293 cells Positive FC (Intra) detected in: Transfected HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins. Specification Tested Reactivity: recombinant protein Cited Reactivity: human, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: MYC tag RRID: AB_2734122 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: MYCTAG Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.