Proteintech, 60052-1-Ig, STOML2 Monoclonal antibody

Size: 20ul / 150ul The STOML2 (60052-1-Ig) by Proteintech is a Monoclonal antibody targeting STOML2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, pig samples 60052-1-Ig targets STOML2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: pig brain tissue, HepG2 cells, MCF-7 cells, RAW 264.7 cells Positive IP detected in: mouse brain tissue Positive IHC detected in: human stomach cancer tissue, human endometrial cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:20000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0363 Product name: Recombinant human STOML2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 82-356 aa of BC002442 Sequence: IVINVPEQSAVTLDNVTLQIDGVLYLRIMDPYKASYGVEDPEYAVTQLAQTTMRSELGKLSLDKVFRERESLNASIVDAINQAADCWGIRCLRYEIKDIHVPPRVKESMQMQVEAERRKRATVLESEGTRESAINVAEGKKQAQILASEAEKAEQINQAAGEASAVLAKAKAKAEAIRILAAALTQHNGDAAASLTVAEQYVSAFSKLAKDSNTILLPSNPGDVTSMVAQAMGVYGALTKAPVPGTPDSLSSGSSRDVQGTDASLDEELDRVKMS Predict reactive species Full Name: stomatin (EPB72)-like 2 Calculated Molecular Weight: 356 aa, 39 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC002442 Gene Symbol: STOML2 Gene ID (NCBI): 30968 RRID: AB_2271235 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9UJZ1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.