Proteintech, 60055-1-Ig, MGP Monoclonal antibody

Size: 20ul / 150ul The MGP (60055-1-Ig) by Proteintech is a Monoclonal antibody targeting MGP in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications with reactivity to human, mouse samples 60055-1-Ig targets MGP in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human placenta tissue, human brain tissue, human kidney tissue, human liver cancer tissue, human saliva Positive IHC detected in: human gliomas tissue, human brain tissue, human lung tissue, human heart tissue, human kidney tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse kidney tissue Positive IF-Fro detected in: mouse brain tissue Positive IF/ICC detected in: H9C2 cells, AC16 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)-FRO: IF-FRO : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Matrix Gla protein (MGP) is is a vitamin K-dependent, extracellular matrix protein. MGP plays a pivotal role in preventing soft tissue calcification and local mineralization of the vascular wall. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites of arterial calcification. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1091 Product name: Recombinant human MGP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-103 aa of BC005272 Sequence: MKSLILLAILAALAVVTLCYESHESMESYELNPFINRRNANTFISPQQRWRAKVQERIRERSKPVHELNREACDDYRLCERYAMVYGYNAAYNRYFRKRRGAK Predict reactive species Full Name: matrix Gla protein Calculated Molecular Weight: 103 aa, 13 kDa Observed Molecular Weight: 12 kDa GenBank Accession Number: BC005272 Gene Symbol: MGP Gene ID (NCBI): 4256 RRID: AB_2143330 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08493 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.