Proteintech, 60085-2-Ig, EEF1D Monoclonal antibody

Size: 20ul / 150ul The EEF1D (60085-2-Ig) by Proteintech is a Monoclonal antibody targeting EEF1D in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 60085-2-Ig targets EEF1D in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: transfection MCF-7 cells, HeLa cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells Positive IP detected in: MCF-7 cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. The calculated molecular weight of EEF1D is a 31 kDa, but the modified protein is about 35-40 kDa. ( PMID: 21936567 ) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0983 Product name: Recombinant human EEF1D protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 347-647 aa of BC007847 Sequence: SHRPGPRSGLSVSSLRPNRKMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI Predict reactive species Full Name: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 35-40 kDa GenBank Accession Number: BC007847 Gene Symbol: EEF1D Gene ID (NCBI): 1936 RRID: AB_2096994 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P29692 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.