Proteintech, 60176-1-Ig, ACPP Monoclonal antibody

Size: 20ul / 150ul The ACPP (60176-1-Ig) by Proteintech is a Monoclonal antibody targeting ACPP in WB, IHC, IF-P, ELISA applications with reactivity to human, rat samples 60176-1-Ig targets ACPP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: K-562 cells, human testis tissue Positive IHC detected in: human prostate hyperplasia tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human prostate cancer tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165). Specification Tested Reactivity: human, rat Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8578 Product name: Recombinant human ACPP protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 27-373 aa of BC007460 Sequence: DRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELAGPVIPQDWSTECM Predict reactive species Full Name: acid phosphatase, prostate Calculated Molecular Weight: 418 aa, 48 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC007460 Gene Symbol: ACPP Gene ID (NCBI): 55 RRID: AB_10733336 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P15309 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.