Proteintech, 60198-1-Ig, RXRA Monoclonal antibody

Size: 20ul / 150ul The RXRA (60198-1-Ig) by Proteintech is a Monoclonal antibody targeting RXRA in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 60198-1-Ig targets RXRA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Hela cells Positive IHC detected in: human stomach cancer tissue, mouse cerebellum tissue, rat liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver, and also expressed in the lungs, kidneys, and heart. It can recognize the the mature 54 kDa RXRA and the truncated 44 kD RXRA (PMID: 20541701). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0987 Product name: Recombinant human RXRA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-165 aa of BC007925 Sequence: MLGSWPARTFHPGACVSRRPSAPWKHTASGKDSPDLRFSEHGVSQEFWAGGLVAVLEMTPSPSPWGTQEGPAGMCSLWVVGWCPCRGAGVRDLVLVHAGVWCKHVCAVQRDACGESRTPAPPRKGGAVTSVLCLFLIKTFPLFSYKFASCKQVHKDPPLVKSGFE Predict reactive species Full Name: retinoid X receptor, alpha Calculated Molecular Weight: 462 aa, 51 kDa Observed Molecular Weight: 44 kDa GenBank Accession Number: BC007925 Gene Symbol: RXRA Gene ID (NCBI): 6256 RRID: AB_10896497 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P19793 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.