Proteintech, 60212-1-Ig, BCS1L Monoclonal antibody

Size: 20ul / 150ul The BCS1L (60212-1-Ig) by Proteintech is a Monoclonal antibody targeting BCS1L in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 60212-1-Ig targets BCS1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Colo320 cells, COLO 320 cells, HEK-293 cells Positive IHC detected in: human gliomas tissue, human brain tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Human BCS1-like (BCS1L), a mitochondrial inner-membrane protein, is a chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Recently studies indicated that the mutations in this protein may cause the GRACILE (growth retardation, aminoaciduria, cholestasis, iron overload, lactacidosis, and early death) syndrome, a recessively inherited lethal disease characterized by fetal growth retardation, lactic acidosis, aminoaciduria, cholestasis, and abnormalities in iron metabolism. Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag18280 Product name: Recombinant human BCS1L protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 7-182 aa of BC007500 Sequence: ILALKDNPYFGAGFGLVGVGTALALARKGVQLGLVAFRRHYMITLEVPARDRSYAWLLSWLTRHSTRTQHLSVETSYLQHESGRISTKFEFVPSPGNHFIWYRGKWIRVERSREMQMIDLQTGTPWESVTFTALGTDRKVFFNILEEARELALQQEEGKTVMYTAVGSEWRPFGYP Predict reactive species Full Name: BCS1-like (yeast) Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC007500 Gene Symbol: BCS1L Gene ID (NCBI): 617 RRID: AB_11042318 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9Y276 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.