Proteintech, 60233-1-Ig, MYH9 Monoclonal antibody

Size: 20ul / 150ul The MYH9 (60233-1-Ig) by Proteintech is a Monoclonal antibody targeting MYH9 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, pig samples 60233-1-Ig targets MYH9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, pig samples. Tested Applications Positive WB detected in: mouse kidney tissue, human kidney tissue, pig spleen tissue, U2OS cells, mouse colon tissue, pig pancreas tissue, A431 cells, human placenta tissue, pig kidney tissue Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156). Specification Tested Reactivity: human, mouse, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1612 Product name: Recombinant human MYH9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1741-1960 aa of BC011915 Sequence: EQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE Predict reactive species Full Name: myosin, heavy chain 9, non-muscle Calculated Molecular Weight: 227 kDa Observed Molecular Weight: 230 kDa, 160 kDa GenBank Accession Number: BC011915 Gene Symbol: MYH9 Gene ID (NCBI): 4627 RRID: AB_2881357 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P35579 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.