Proteintech, 60255-1-Ig, SMN-Exon7 Monoclonal antibody

Size: 20ul / 150ul The SMN-Exon7 (60255-1-Ig) by Proteintech is a Monoclonal antibody targeting SMN-Exon7 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 60255-1-Ig targets SMN-Exon7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells Positive IHC detected in: mouse heart tissue, mouse brain tissue, mouse pancreas tissue, rat brain tissue, rat heart tissue, rat pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 60255-1-Ig, raised against the C-terminal region (275-294aa) encoded by the exon 7. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16615 Product name: Recombinant human SMN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 275-294 aa of BC062723 Sequence: GYYMGFRQNQKEGRCSHSLN Predict reactive species Full Name: survival of motor neuron 1, telomeric Calculated Molecular Weight: 294 aa, 32 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC062723 Gene Symbol: SMN Gene ID (NCBI): 6606 RRID: AB_2881376 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q16637 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.