Proteintech, 60259-2-Ig, Napsin A Monoclonal antibody

Size: 20ul / 150ul The Napsin A (60259-2-Ig) by Proteintech is a Monoclonal antibody targeting Napsin A in IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 60259-2-Ig targets Napsin A in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human lung cancer tissue, human kidney tissue, human renal cell carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HUVEC cells Positive FC (Intra) detected in: A549 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:10000-1:60000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9721 Product name: Recombinant human NAPSA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-420 aa of BC017842 Sequence: MSPPPLLQPLLLLLPLLNVEPSGATLIRIPLHRVQPGRRTLNLLRGWREPAELPKLGAPSPGDKPIFVPLSNYRDVQYFGEIGLGTPPQNFTVAFDTGSSNLWVPSRRCHFFSVPCWLHHRFDPKASSSFQANGTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVFAFAHFDGILGLGFPILSVEGVRPPMDVLVEQGLLDKPVFSFYLNRDPEEPDGGELVLGGSDPAHYIPPLTFVPVTVPAYWQIHMERVKVGPGLTLCAKGCAAILDTGTSLITGPTEEIRALHAAIGGIPLLAGEYIILCSEIPKLPAVSFLLGGVWFNLTAHDYVIQTTRNGVRLCLSGFQALDVPPPAGPFWILGDVFLGTYVAVFDRGDMKSSARVGLARARTRGADLGWGETAQAQFPG Predict reactive species Full Name: napsin A aspartic peptidase Calculated Molecular Weight: 420 aa, 45 kDa GenBank Accession Number: BC017842 Gene Symbol: Napsin A Gene ID (NCBI): 9476 RRID: AB_2881381 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O96009 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.