Proteintech, 60265-1-Ig, SND1 Monoclonal antibody

Size: 20ul / 150ul The SND1 (60265-1-Ig) by Proteintech is a Monoclonal antibody targeting SND1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 60265-1-Ig targets SND1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells, U2OS cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, A431 cells, LNCaP cells Positive IP detected in: HeLa cells Positive IHC detected in: human pancreas tissue, human breast hyperplasia tissue, human breast cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information Staphylococcal nuclease domain-containing 1 (SND1), is a multifunctional nuclease that consists of four staphylococcal nuclease domains and a tudor domain. SND1 acts as a coactivator that facilitates transcriptional activity of STAT5, 6 and c-Myc. SND1 is a comprising part of the RNA-induced silencing complex(RISC), and takes part in the functions of miRNA, regulates transcription through transcriptional coactivation, RNA interference, RNA splicing, and RNA editing. Higher level of SND1 has been found in colon cancer and prostate cancer, can promote HCC angiogenesis in xenograft model through induction of angiogenic factors. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1200 Product name: Recombinant human SND1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 548-911 aa of BC017180 Sequence: TCLITFLLAGIECPRGARNLPGLVQEGEPFSEEATLFTKELVLQREVEVEVESMDKAGNFIGWLHIDGANLSVLLVEHALSKVHFTAERSSYYKSLLSAEEAAKQKKEKVWAHYEEQPVEEVMPVLEEKERSASYKPVFVTEITDDLHFYVQDVETGTQLEKLMENMRNDIASHPPVEGSYAPRRGEFCIAKFVDGEWYRARVEKVESPAKIHVFYIDYGNREVLPSTRLGTLSPAFSTRVLPAQATEYAFAFIQVPQDDDARTDAVDSVVRDIQNTQCLLNVEHLSAGCPHVTLQFADSKGDVGLGLVKEGLVMVEVRKEKQFQKVITEYLNAQESAKSARLNLWRYGDFRADDADEFGYSR Predict reactive species Full Name: staphylococcal nuclease and tudor domain containing 1 Calculated Molecular Weight: 101 kDa Observed Molecular Weight: 101 kDa GenBank Accession Number: BC017180 Gene Symbol: SND1 Gene ID (NCBI): 27044 RRID: AB_2881386 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q7KZF4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.