Proteintech, 60277-1-Ig, Chk1 Monoclonal antibody

Size: 20ul / 150ul The Chk1 (60277-1-Ig) by Proteintech is a Monoclonal antibody targeting Chk1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 60277-1-Ig targets Chk1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, K-562 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:3000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0409 Product name: Recombinant human CHK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 42-256 aa of BC004202 Sequence: MKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITI Predict reactive species Full Name: CHK1 checkpoint homolog (S. pombe) Calculated Molecular Weight: 54 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC004202 Gene Symbol: Chk1 Gene ID (NCBI): 1111 RRID: AB_2881396 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14757 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.