Proteintech, 60309-1-Ig, pan Ras Monoclonal antibody

Size: 20ul / 150ul The pan Ras (60309-1-Ig) by Proteintech is a Monoclonal antibody targeting pan Ras in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat, pig, rabbit, chicken samples 60309-1-Ig targets pan Ras in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples. Tested Applications Positive WB detected in: fetal human brain tissue, pig brain tissue, HEK-293 cells, Neuro-2a cells, ROS1728 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, human brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human lung cancer tissue, human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:20000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras. Specification Tested Reactivity: human, mouse, rat, pig, rabbit, chicken Cited Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag2700 Product name: Recombinant human KRAS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC013572 Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM Predict reactive species Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Calculated Molecular Weight: 188 aa, 21 kDa Observed Molecular Weight: 21 kDa GenBank Accession Number: BC013572 Gene Symbol: KRAS Gene ID (NCBI): 3845 RRID: AB_2881422 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P01116 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.