Proteintech, 60330-1-Ig, Vimentin Monoclonal antibody

Size: 20ul / 150ul The Vimentin (60330-1-Ig) by Proteintech is a Monoclonal antibody targeting Vimentin in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 60330-1-Ig targets Vimentin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, NIH/3T3 cells, ROS1728 cells, Jurkat cells, A549 cells, U2OS cells, U-251 cells Positive IHC detected in: human appendicitis tissue, human breast cancer tissue, human colon tissue, human endometrial cancer tissue, human liver cancer tissue, human ovary tumor tissue, human pancreas tissue, human renal cell carcinoma tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: Customer Sample customer Positive IF/ICC detected in: HUVEC cells, HepG2 cells, Retinal organoids Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunohistochemistry (IHC): IHC : 1:4000-1:16000 Immunofluorescence (IF)-P: IF-P : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, rabbit, zebrafish Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag0489 Product name: Recombinant human Vimentin protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-466 aa of BC000163 Sequence: MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE Predict reactive species Full Name: vimentin Calculated Molecular Weight: 466 aa, 54 kDa Observed Molecular Weight: 55-60 kDa GenBank Accession Number: BC000163 Gene Symbol: VIM Gene ID (NCBI): 7431 ENSEMBL Gene ID: ENSG00000026025 RRID: AB_2881439 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P08670 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.