Proteintech, 60423-1-Ig, BUB1B Monoclonal antibody

Size: 20ul / 150ul The BUB1B (60423-1-Ig) by Proteintech is a Monoclonal antibody targeting BUB1B in WB, ELISA applications with reactivity to human samples 60423-1-Ig targets BUB1B in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HaCaT cells, Saos-2 cells, U2OS cells, HeLa cells, HEK-293 cells, Ramos cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1). Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag34182 Product name: Recombinant human BUB1B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 719-1050 aa of BC018739 Sequence: QSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ Predict reactive species Full Name: budding uninhibited by benzimidazoles 1 homolog beta (yeast) Calculated Molecular Weight: 1050 aa, 120 kDa Observed Molecular Weight: 120-130 kDa GenBank Accession Number: BC018739 Gene Symbol: BubR1 Gene ID (NCBI): 701 RRID: AB_3670258 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O60566 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.