Proteintech, 60479-1-Ig, Phospho-STAT3 (Ser727) Monoclonal antibody

Size: 20ul / 100ul The Phospho-STAT3 (Ser727) (60479-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-STAT3 (Ser727) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 60479-1-Ig targets Phospho-STAT3 (Ser727) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, A549 cells, UV treated HSC-T6 cells, MCF-7 cells, NIH/3T3 cells, EGF treated A549 cells, UV treated HeLa cells, TNF alpha treated MCF-7 cells Positive IF/ICC detected in: UV(1hour),100 nM Calyculin A(30 minutes)treated HeLa cells, HeLa cells Positive FC (Intra) detected in: UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension Background Information Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: signal transducer and activator of transcription 3 (acute-phase response factor) Calculated Molecular Weight: 770 aa, 88 kDa Observed Molecular Weight: 85-90 kDa GenBank Accession Number: BC000627 Gene Symbol: STAT3 Gene ID (NCBI): 6774 RRID: AB_3670262 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P40763 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.