Proteintech, 60555-1-Ig, Cleaved PARP1 Monoclonal antibody

Size: 20ul / 150ul The Cleaved PARP1 (60555-1-Ig) by Proteintech is a Monoclonal antibody targeting Cleaved PARP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 60555-1-Ig targets Cleaved PARP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A2780 cells, HSC-T6 cells, mouse splenocytes, Staurosporine treated A2780 cells, Staurosporine treated HSC-T6 cells, Staurosporine treated mouse splenocytes Positive IHC detected in: Jurkat cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: 1 μM Staurosporine (3 hours) treated HSC-T6 cells, 1 μM Staurosporine (3 hours) treated HeLa cells Positive FC (Intra) detected in: 1 μM  Staurosporine (3 hours) treated HSC-T6 cells, 1 μM  Staurosporine (3 hours) treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: poly (ADP-ribose) polymerase 1 Calculated Molecular Weight: 1014 aa, 113 kDa Observed Molecular Weight: 89 kDa GenBank Accession Number: BC037545 Gene Symbol: PARP1 Gene ID (NCBI): 142 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P09874 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.