Proteintech, 60666-1-Ig, Phospho-JNK (Thr183/Tyr185) Monoclonal antibody

Size: 20ul / 100ul The Phospho-JNK (Thr183/Tyr185) (60666-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-JNK (Thr183/Tyr185) in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 60666-1-Ig targets Phospho-JNK (Thr183/Tyr185) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, NIH/3T3 cells, HSC-T6 cells, UV treated HEK-293 cells, UV treated NIH/3T3 cells, UV treated HSC-T6 cells, Anisomycin treated HeLa cells Positive IHC detected in: Jurkat cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:1000 Background Information MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, chicken Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mitogen-activated protein kinase 8 Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 42 kDa, 50 kDa GenBank Accession Number: NM_138982 Gene Symbol: JNK Gene ID (NCBI): 5599 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P45983 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.