Proteintech, 60875-5-Ig, Ferritin heavy chain Monoclonal antibody

Size: 20ul / 150ul The Ferritin heavy chain (60875-5-Ig) by Proteintech is a Monoclonal antibody targeting Ferritin heavy chain in WB, IHC, IF/ICC, ELISA applications with reactivity to human, rat, rabbit samples 60875-5-Ig targets Ferritin heavy chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, rabbit samples. Tested Applications Positive WB detected in: A549 cells, K-562 cells, rabbit brain tissue, U-251 cells, HeLa cells, rat brain tissue, rabbit heart tissue, PC-3 cells, HaCaT cells Positive IHC detected in: rat liver tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis. Specification Tested Reactivity: human, rat, rabbit Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag21386 Product name: Recombinant human FTH1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-183 aa of BC000857 Sequence: MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES Predict reactive species Full Name: ferritin, heavy polypeptide 1 Calculated Molecular Weight: 183 aa, 21 kDa Observed Molecular Weight: 21 kDa GenBank Accession Number: BC000857 Gene Symbol: FTH1 Gene ID (NCBI): 2495 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P02794 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.