Proteintech, 65051-1-Ig, Anti-Human CD107a / LAMP1 (H4A3)

Size: 100ug / 500ug The CD107a / LAMP1 (65051-1-Ig) by Proteintech is a Monoclonal antibody targeting CD107a / LAMP1 in WB, IF/ICC, FC (Intra) applications with reactivity to human samples 65051-1-Ig targets CD107a / LAMP1 in WB, IF/ICC, FC (Intra) applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension Background Information LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697). Specification Tested Reactivity: human Cited Reactivity: human, mouse, monkey Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: Human adherent peripheral blood cells Predict reactive species Full Name: lysosomal-associated membrane protein 1 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 100-120 kDa GenBank Accession Number: BC006345 Gene Symbol: LAMP1 Gene ID (NCBI): 3916 ENSEMBL Gene ID: ENSG00000185896 RRID: AB_2881467 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P11279 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.