Proteintech, 65069-1-Ig, Anti-Mouse CD8a (53-6.7)

Size: 500ug The CD8a (65069-1-Ig) by Proteintech is a Monoclonal antibody targeting CD8a in FC applications with reactivity to mouse samples 65069-1-Ig targets CD8a in FC applications and shows reactivity with mouse samples. Tested Applications Positive IF-P detected in: mouse spleen tissue Positive FC detected in: mouse splenocytes Recommended dilution Immunofluorescence (IF)-P: IF-P : 1:400-1:1600 Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer, while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084). Specification Tested Reactivity: mouse Cited Reactivity: mouse Host / Isotype: Rat / IgG2a, kappa Class: Monoclonal Type: Antibody Immunogen: Spleen cells or thymocyte membranes Predict reactive species Full Name: CD8 antigen, alpha chain GenBank Accession Number: BC030679 Gene Symbol: Cd8a Gene ID (NCBI): 12525 RRID: AB_2881470 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P01731 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.