Proteintech, 65077-1-Ig, Anti-Mouse CD3 (17A2)

Size: 500ug The CD3 (65077-1-Ig) by Proteintech is a Monoclonal antibody targeting CD3 in FC applications with reactivity to mouse samples 65077-1-Ig targets CD3 in FC, FC (Intra) applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: C57BL/6 mouse splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). Specification Tested Reactivity: mouse Cited Reactivity: mouse Host / Isotype: Rat / IgG2b, kappa Class: Monoclonal Type: Antibody Immunogen: γδTCR-positive T-T hybridoma D1 Predict reactive species Full Name: CD3 antigen, epsilon polypeptide GenBank Accession Number: BC098236 Gene Symbol: CD3ε Gene ID (NCBI): 12501 RRID: AB_2918381 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P22646 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.