Proteintech, 65089-1-Ig, Anti-Mouse Foxp3 (3G3)

Size: 100ug / 500ug The Foxp3 (65089-1-Ig) by Proteintech is a Monoclonal antibody targeting Foxp3 in IHC, FC (Intra) applications with reactivity to Mouse samples 65089-1-Ig targets Foxp3 in IHC, IF, FC (Intra) applications and shows reactivity with Mouse samples. Tested Applications Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: mouse splenocytes Positive FC detected in: mouse splenocytes Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension Flow Cytometry (FC): FC : Background Information Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993). Specification Tested Reactivity: Mouse Cited Reactivity: mouse Host / Isotype: Mouse / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: N/A Predict reactive species Full Name: forkhead box P3 GenBank Accession Number: BC132333 Gene Symbol: Foxp3 Gene ID (NCBI): 20371 RRID: AB_2918392 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: Q99JB6 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.