Proteintech, 65137-1-Ig, Anti-Mouse CD25/IL-2RA (PC61.5)

Size: 500ug The CD25/IL-2RA (65137-1-Ig) by Proteintech is a Monoclonal antibody targeting CD25/IL-2RA in FC applications with reactivity to mouse samples 65137-1-Ig targets CD25/IL-2RA in FC applications and shows reactivity with mouse samples. Tested Applications Positive FC detected in: Anti-CD3 and Anti-CD28 stimulated mouse splenocytes Recommended dilution Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674). Specification Tested Reactivity: mouse Host / Isotype: Rat / IgG1, lambda Class: Monoclonal Type: Antibody Immunogen: N/A Predict reactive species Full Name: interleukin 2 receptor, alpha chain GenBank Accession Number: BC114437 Gene Symbol: CD25 Gene ID (NCBI): 16184 RRID: AB_2918424 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P01590 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.