Proteintech, 65139-1-Ig, Anti-Mouse CD45R (B220) (RA3-6B2)

Size: 500ug The CD45R (B220) (65139-1-Ig) by Proteintech is a Monoclonal antibody targeting CD45R (B220) in IHC, IF/ICC, IF-P, FC applications with reactivity to mouse samples 65139-1-Ig targets CD45R (B220) in IHC, IF/ICC, IF-P, FC applications and shows reactivity with mouse samples. Tested Applications Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse spleen tissue Positive IF/ICC detected in: mouse splenocytes Positive FC detected in: mouse splenocytes Recommended dilution Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension Background Information CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R. Specification Tested Reactivity: mouse Cited Reactivity: mouse Host / Isotype: Rat / IgG2a, kappa Class: Monoclonal Type: Antibody Immunogen: Abelson murine leukemia virus-induced pre-B tumor cells Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, C GenBank Accession Number: BC028512 Gene Symbol: CD45 Gene ID (NCBI): 19264 RRID: AB_2918426 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P06800 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.