Proteintech, 65153-1-Ig, Anti-Mouse IFN-gamma (XMG1.2)

Size: 500ug The IFN-gamma (65153-1-Ig) by Proteintech is a Monoclonal antibody targeting IFN-gamma in FC (Intra) applications with reactivity to mouse samples 65153-1-Ig targets IFN-gamma in FC (Intra) applications and shows reactivity with mouse samples. Tested Applications Positive FC (Intra) detected in: PMA and ionomycin stimulated C57BL/6 Th1-polarized splenocytes Recommended dilution Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension Background Information Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427) Specification Tested Reactivity: mouse Host / Isotype: Rat / IgG1, kappa Class: Monoclonal Type: Antibody Immunogen: Purified Recombinant Mouse IFN gamma Predict reactive species Full Name: interferon gamma Calculated Molecular Weight: 17 kDa GenBank Accession Number: BC119063 Gene Symbol: IFN gamma Gene ID (NCBI): 15978 RRID: AB_3670276 Conjugate: Unconjugated Form: Liquid Purification Method: Affinity purification UNIPROT ID: P01580 Storage Buffer: PBS with 0.09% sodium azide, pH 7.3. Storage Conditions: Store at 2-8°C. Stable for one year after shipment.